Catalog Number - 65318-10P-2

Zx Similars C18™ is a traditional reversed-phase HPLC stationary phase manufactured on high-purity Type-B spherical silica with a fully endcapped octadecyl (C18) bonded phase. Designed as a cost-effective alternative to Agilent Zorbax® C18 columns, it offers comparable chromatographic behavior for USP L1 methods while providing the strong hydrophobic retention, reproducibility, and broad applicability expected from a conventional C18 stationary phase.

65318-10P-2 is a 100 mm x 2.1 mm ID narrow-bore analytical column packed with 3 µm Zx Similars C18 particles. The 100 mm length provides an excellent balance of chromatographic resolution and analysis speed, while the 2.1 mm ID format minimizes solvent consumption and is particularly well suited for LC-MS applications. The 3 µm particle size delivers higher efficiency and improved peak capacity than traditional 5 µm columns, although it generates increased operating pressure. Typical operating flow rates are approximately 0.2–0.3 mL/min and require an HPLC or LC-MS system capable of reliable low-flow operation with low-volume plumbing and flow cells.

This USP L1 column is ideal for hydrophobic and neutral compounds requiring strong reversed-phase retention. Its narrow-bore design, low-flow operation, and enhanced efficiency make it a popular choice for LC-MS methods, pharmaceutical analysis, long-chain fatty acid separations, and high-sensitivity analytical workflows requiring chromatographic behavior similar to Agilent Zorbax® C18 columns.

Key Benefits

• Type-B spherical silica stationary phase

• Suitable alternative for USP L1 methods

• Comparable selectivity to Agilent Zorbax® C18 columns

• Narrow-bore format ideal for LC-MS applications

• Balanced speed and resolution with 100 mm length

• Higher efficiency than comparable 5 µm columns

• Reduced solvent consumption

• End-capped C18 bonded phase

Typical Applications

• USP L1 methods

• LC-MS analysis

• Reversed-phase HPLC

• Pharmaceutical analysis

• Long-chain fatty acid analysis

• Quality control testing

• Method development

• Method transfer and validation

Specifications

• Product Type: HPLC Column

• Stationary Phase: Zx Similars C18

• USP Classification: L1

• Particle Size: 3 µm

• Particle Type: Type-B Spherical Silica

• Bonded Phase: C18 (Octadecyl)

• Endcapped: Yes

• Column Dimensions: 2.1 mm ID x 100 mm

• Length: 100 mm

• Recommended Flow Rate: 0.2–0.3 mL/min

• Brand: Cogent

Important Notes

• Designed for reversed-phase chromatography

• Narrow-bore format optimized for LC-MS applications

• Requires an HPLC or LC-MS system capable of reliable low-flow operation

• Higher efficiency than comparable 5 µm columns

• Increased operating pressure compared to larger particle sizes

• Reduced solvent consumption compared to 4.6 mm ID columns

• Excellent alternative for many USP L1 methods

• Ideal for high-sensitivity LC-MS workflows

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